Amino acid sequence of the peptostreptococcus elsdenii flavodoxin

Electron-nuclear double resonance study of flavodoxin from Peptostreptococcus elsdenii.

Peptostreptococcus elsdenii from the caecum of pigs.

Large, Gram-negative, anaerobic cocci (LC) fermenting lactate were first isolated by enrichment culture from the sheep rumen (Elsden, Volcani, Gilchrist & Lewis, 1956). High counts (about 109/ml.) were obtained from young calves but not from adult ruminants by Hobson, Mann & Oxford (1958). The cocci were cultured in numbers of 1 0 6 to 103/ml. from the rumen of cattle fed high-grain rations and...

Dependence of the proton magnetic resonance spectra on the oxidation state of flavodoxin from Clostridium MP and from Peptostreptococcus elsdenii.

The broadening of protein nuclear magnetic resonances in the spectra of the semiquinone forms of flavodoxins derived from Clostridium MP and Peptostreptococcus elsdenii relative to the resonances in the oxidized and reduced forms is highly selective. Spectra from both species of flavodoxin indicate that conformational differences between the oxidized and fully reduced states are minor and, cons...

Amino acid sequences of Clostridium pasteurianum flavodoxin.

Lys 10 10 8 4 8 7 5 12 9 13 9 Try 4 4 4 1 2 1 6 3 4 3 4 6 His 0 0 0 1 0 3 I 4 1 0 0 Arg 2 2 2 6 3 3 4 4 8 6 2 Asp 18 17 16 20 17 1 3 1 4 22 28 25 21 28 Thr 4 5 8 6 9 6 8 14 16 8 8 Ser 14 8 7 7 8 4 4 11 11 15 12 Glu 19 21 18 17 18 1 6 1 7 25 22 16 23 26 Pro 4 4 5 4 6 3 4 5 7 5 3 Gly 17 14 14 19 14 11 19 25 28 21 19 Ala 14 6 18 18 15 11 15 24 20 15 12 CvS 1 3 2 5 5 3 2 5 2 1 1 Val 15 10 13 10 16 ...

Purification and properties of electron-transferring flavoprotein from Peptostreptococcus elsdenii.

An electron-transferring flavoprotein (ETF) which couples the oxidation of NADH to the reduction of butyryl coenzyme A dehydrogenase has been purified in large quantities from the anaerobic bacterium Peptostreptococcus elsdenii. The purified protein appears to be homogeneous on the basis of polyacrylamide disc gel electrophoresis, thin layer isoelectric focusing, sedimentation velocity, and the...